In amperometric glucose sensors, which species is oxidized at the electrode to generate the current signal?

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Study for the Ciulla Clinical Chemistry Test. Enhance your knowledge with flashcards and multiple-choice questions. Prepare for the exam with comprehensive study materials and detailed explanations for each question.

Multiple Choice

In amperometric glucose sensors, which species is oxidized at the electrode to generate the current signal?

Explanation:
In amperometric glucose sensing with glucose oxidase, glucose is first oxidized by the enzyme, which reduces oxygen to hydrogen peroxide. The hydrogen peroxide then diffuses to the electrode and is oxidized there, producing the measurable current. The electrode reaction is H2O2 → O2 + 2H+ + 2e−, so the current corresponds to the amount of H2O2 formed, linking it to the glucose concentration.

In amperometric glucose sensing with glucose oxidase, glucose is first oxidized by the enzyme, which reduces oxygen to hydrogen peroxide. The hydrogen peroxide then diffuses to the electrode and is oxidized there, producing the measurable current. The electrode reaction is H2O2 → O2 + 2H+ + 2e−, so the current corresponds to the amount of H2O2 formed, linking it to the glucose concentration.

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