In the cholesterol oxidase-peroxidase method, cholesterol oxidase reacts with which form?

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Study for the Ciulla Clinical Chemistry Test. Enhance your knowledge with flashcards and multiple-choice questions. Prepare for the exam with comprehensive study materials and detailed explanations for each question.

Multiple Choice

In the cholesterol oxidase-peroxidase method, cholesterol oxidase reacts with which form?

Explanation:
Cholesterol oxidase specifically acts on free cholesterol. In the cholesterol oxidase–peroxidase assay, any cholesteryl esters in the sample must first be hydrolyzed by cholesterol esterase to free cholesterol; only then is the cholesterol oxidase able to oxidize it and generate hydrogen peroxide. That hydrogen peroxide is then used by peroxidase to produce a colored product proportional to the cholesterol concentration. So the form that cholesterol oxidase reacts with is free cholesterol, not cholesteryl esters.

Cholesterol oxidase specifically acts on free cholesterol. In the cholesterol oxidase–peroxidase assay, any cholesteryl esters in the sample must first be hydrolyzed by cholesterol esterase to free cholesterol; only then is the cholesterol oxidase able to oxidize it and generate hydrogen peroxide. That hydrogen peroxide is then used by peroxidase to produce a colored product proportional to the cholesterol concentration. So the form that cholesterol oxidase reacts with is free cholesterol, not cholesteryl esters.

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