In the hexokinase-based glucose assay, which products are formed?

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Study for the Ciulla Clinical Chemistry Test. Enhance your knowledge with flashcards and multiple-choice questions. Prepare for the exam with comprehensive study materials and detailed explanations for each question.

Multiple Choice

In the hexokinase-based glucose assay, which products are formed?

Explanation:
The main idea is that hexokinase transfers a phosphate from ATP to glucose, producing glucose-6-phosphate and ADP. In this first step of the assay, ATP donates its terminal phosphate to glucose, so the products are glucose-6-phosphate (G6P) and ADP. In typical glucose assays, the formed G6P is then used in the next enzyme step to reduce NADP+ to NADPH, which is what’s measured. So the immediate products of the hexokinase reaction are glucose-6-phosphate and ADP; NADPH (or NADH) appears only in the subsequent coupled reaction, not in the initial step.

The main idea is that hexokinase transfers a phosphate from ATP to glucose, producing glucose-6-phosphate and ADP. In this first step of the assay, ATP donates its terminal phosphate to glucose, so the products are glucose-6-phosphate (G6P) and ADP. In typical glucose assays, the formed G6P is then used in the next enzyme step to reduce NADP+ to NADPH, which is what’s measured. So the immediate products of the hexokinase reaction are glucose-6-phosphate and ADP; NADPH (or NADH) appears only in the subsequent coupled reaction, not in the initial step.

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