Which colorimetric method is widely used for salicylate determination?

Which colorimetric method is widely used for salicylate determination?

• A. Trinder reaction
• B. Benedict's test
• C. Biuret method
• D. Jaffe reaction

Answer: (A)

The key idea is a peroxidase-driven colorimetric reaction that links the amount of salicylate to a colored product. In this approach, an enzyme converts salicylate into a compound that releases hydrogen peroxide. That hydrogen peroxide then powers the Trinder reaction, where a phenol and 4-aminophenazone are oxidized by peroxidase in the presence of hydrogen peroxide to form a pink-to-red dye. The intensity of this color, read spectrophotometrically, grows with the salicylate concentration, giving a sensitive and robust measurement. This method is favored because it is simple, fast, and compatible with automation, providing reliable results over a wide range of concentrations. By comparison, the other tests are designed for different targets—reducing sugars, proteins, or creatinine—so they aren’t used for salicylate determination.

The key idea is a peroxidase-driven colorimetric reaction that links the amount of salicylate to a colored product. In this approach, an enzyme converts salicylate into a compound that releases hydrogen peroxide. That hydrogen peroxide then powers the Trinder reaction, where a phenol and 4-aminophenazone are oxidized by peroxidase in the presence of hydrogen peroxide to form a pink-to-red dye. The intensity of this color, read spectrophotometrically, grows with the salicylate concentration, giving a sensitive and robust measurement. This method is favored because it is simple, fast, and compatible with automation, providing reliable results over a wide range of concentrations. By comparison, the other tests are designed for different targets—reducing sugars, proteins, or creatinine—so they aren’t used for salicylate determination.